Long-term preservation protocol for filamentous fungi

Penicillium roqueforti

A conidial suspension of sporulating filamentous fungi is prepared in an appropriate cryoprotectant solution such as DMSO or glycerol / skim milk. A stepwise freezing rate to minimize intracellular ice crystal formation is used. The samples are cooled very rapidly from a given temperature and stored in the gas phase of liquid nitrogen.

Fungal cultures that do not sporulate can also be stored in the gas phase of liquid nitrogen. Mycelia containing plugs cut from agar cultures are placed in vials containing glycerol and stored at minus 196°C.

CCOS offers a cryopreservation service to ensure the long-term viability and genetic stability of your filamentous fungi cultures, used in biotechnology for the production of fermented foods, enzymes, drugs and chemicals. Do not hesitate to contact us for further information.