Pseudomonas / E. coli shuttle vectors

Pseudomonas cloning vectors which contain the minimal replicon of the plasmid pVS1 and the E. coli replicon p15A are stable in Pseudomonas fluorescens/protegens. The plasmid pME6012 showed an elevated copy numer of about 15 copies per Pseudomonas fluorescens/protegens cell due to a mutation in the repA gene. Pseudomonas protegens CHA0 (CCOS 2) can be used as host strain. 

(PMID: 10659714, PMID: 11807065, Depositor: Dieter Haas).